白癜风治疗价格 http://pf.39.net/bdfyy/bdfjc/150526/4629682.htmlRegenerationofafull-thicknessdefectofrotatorcufftendonwithfreshlythawedumbilicalcord-derivedmesenchymalstemcellsinaratmodel
新鲜解冻脐带间充质干细胞用于大鼠肩袖肌腱全层缺损再生
作者：YeaJH,ParkJK,KimIJ,SymG,BaeTS,JoCH.
期刊：StemCellResearchandTherapy
日期：.09
DOI：10./s---1
IF：5.12
Background
Itisdifficulttoimmediatelyusemesenchymalstemcells(MSCs)forthepatientwithrotatorcuffdiseasebecauseisolationandculturetimearerequired.Thus,theMSCswouldbepreparedinadvancedincryopreservedconditionforan"off-the-shelf"usageinclinic.ThisstudyinvestigatedtheefficacyoffreshlythawedMSCsontheregenerationofafull-thicknesstendondefect(FTD)ofrotatorcufftendoninaratmodel.
间充质干细胞（mesenchymalstemcells，MSCs）在肩袖病患者中的应用由于需要分离和培养时间而难以立即应用。因此，骨髓间充质干细胞将在低温保存条件下提前制备，以供临床“现成”使用。本研究探讨新鲜解冻的间充质干细胞在大鼠肩袖肌腱全层缺损（FTD）再生中的作用。
Methods
Weevaluatedmorphology,viability,andproliferationofculturedumbilicalcord-derivedMSCs(C-UCMSCs)andfreshlythawedumbilicalcord-derivedMSCs(T-UCMSCs)atpassage10invitro.Inanimalexperiments,wecreatedaFTDinthesupraspinatusofratsandinjectedtheinjuredtendonwithsaline,cryopreservedagent(CPA;control),C-UCMSCs,andT-UCMSCs,respectively.Twoand4weekslater,macroscopic,histological,biomechanical,andcelltraffickingwereevaluated.TtestandANOVAwereusedwithSPSS.Differenceswithp.05wereconsideredstatisticallysignificant.
观察体外培养的脐带源性MSCs（C-UC-MSCs）和新鲜解冻的脐带源性MSCs（T-UC-MSCs）在体外培养第10代时的形态、活性和增殖能力。在动物实验中，我们在大鼠的冈上肌上建立一个FTD，分别注入生理盐水、冷冻保存剂（CPA；对照）、C-UC-MSCs和T-UC-MSCs。2周和4周后，进行宏观、组织学、生物力学和细胞运输的评估。用SPSS软件进行T检验和方差分析。p0.05的差异被认为具有统计学意义。
Results
T-UCMSCshadfibroblast-likemorphologyandshowedgreaterthan97%viabilityandstableproliferation
